Welcome to the Michel Lab Website!
My lab explores signal transduction pathways in the cells and tissues of the cardiovascular system. We have a particular focus on applying biochemical methods and cell imaging approaches using informative biosensors to probe nitric oxide synthase signaling pathways and their interactions with reactive oxygen species in endothelial cells and cardiac myocytes, studying cultured cell systems, animal models of cardiovascular disease states, and human cells and tissues.
Some of our recent studies have exploited novel biosensors in cellular imaging studies to explore the relationship between nitric oxide and reactive oxygen species. For example
- We have been studying receptor-modulated H2O2 metabolism using live cell imaging approaches both in cardiac myocytes and in endothelial cells.
- We have also been exploring the roles of “statin” drugs (HMG CoA reductase inhibitors commonly used in the treatment of cardiovascular disease) in eNOS and H2O2 signaling
- We discovered that Rac1 and H2O2 regulate the MARCKS protein, an enigmatic actin-binding protein that plays a key role in regulation of endothelial permeability.
- We are also studying the role of NO and H2O2 in regulating the AMP-activated protein kinase (AMPK) and other kinases involved in eNOS signaling in endothelial cells and in the heart, with a particular interest in the perturbations of these pathways in diabetes and in response to oxidative stress.