PUBLICATIONS

Here you can find some of our recent publications. To see more of our work go to original articles or selected reviews

  • Yadav S, Waldeck-Weiermair M, Spyropoulos F, Bronson R, Pandey AK, Das AA, Sisti AC, Covington TA, Thulabandu V, Caplan S, Chutkow W, Steinhorn B, Michel T. Sensory ataxia and cardiac hypertrophy caused by neurovascular oxidative stress in chemogenetic transgenic mouse lines. Nat Commun. 2023 May 29;14(1):3094. doi: 10.1038/s41467-023-38961-0. PMID: 37248315; PMCID: PMC10227029.
  • Steinhorn B, Eroglu E, Michel T. Chemogenetic Approaches to Probe Redox Pathways: Implications for Cardiovascular Pharmacology and Toxicology. Annu Rev Pharmacol Toxicol. 2022 Jan 6;62:551-571. doi: 10.1146/annurev-pharmtox-012221-082339. Epub 2021 Sep 16. PMID: 34530645. PubMed link
  • Spyropoulos F, Sorrentino A, van der Reest J, Yang P, Waldeck-Weiermair M, Steinhorn B, Eroglu E, Saeedi Saravi SS, Yu P, Haigis M, Christou H, Michel T. Metabolomic and transcriptomic signatures of chemogenetic heart failure. Am J Physiol Heart Circ Physiol. 2022 Mar 1;322(3):H451-H465. doi: 10.1152/ajpheart.00628.2021. Epub 2022 Jan 28. PMID: 35089810. PubMed link
  • Waldeck-Weiermair M, Yadav S, Spyropoulos F, Krüger C, Pandey AK, Michel T. Dissecting in vivo and in vitro redox responses using chemogenetics. Free Radic Biol Med. 2021 Dec;177:360-369. doi: 10.1016/j.freeradbiomed.2021.11.006. Epub 2021 Nov 6. PMID: 34752919; PMCID: PMC8639655.   PubMed link
  • Erdogan YC, Altun HY, Secilmis M, Ata BN, Sevimli G, Cokluk Z, Zaki AG, Sezen S, Akgul Caglar T, Sevgen İ, Steinhorn B, Ai H, Öztürk G, Belousov VV, Michel T, Eroglu E. Complexities of the chemogenetic toolkit: Differential mDAAO activation by d-amino substrates and subcellular targeting. Free Radic Biol Med. 2021 Dec;177:132-142. doi: 10.1016/j.freeradbiomed.2021.10.023. Epub 2021 Oct 20. PMID: 34687864; PMCID: PMC8639799.  PubMed link
  • Saeedi Saravi SS, Eroglu E, Waldeck-Weiermair M, Sorrentino A, Steinhorn B, Belousov V, Michel T. Differential endothelial signaling responses elicited by chemogenetic H2O2 synthesis. Redox Biol. 2020 Sep;36:101605. doi: 10.1016/j.redox.2020.101605. Epub 2020 Jun 16. PMID: 32590330; PMCID: PMC7322171.  PubMed link
  • Pak VV…Michel T, Vriz S, Messens J, Belousov VV. Ultrasensitive genetically encoded indicator for intracellular hydrogen peroxide identifies novel roles for cellular oxidants in cell migration and mitochondrial function. Cell Metabolism 2020, 31:642-653. PubMed link
  • Sorrentino A and Michel T. Redox a la Carte: Novel chemogenetic models of heart failure.  British Journal of Pharmacology 2020, DOI: 10.1111/bph.15093. PubMed link
  • Saravi S, Eroglu E, Waldeck-Weiermair M, Sorrentino A, Steinhorn B, Belousov V, Michel T. Differential endothelial signaling responses elicited by chemogenetic hydrogen peroxide synthesis.  Redox Biology 2020, 36:101605, https://doi.org/10.1016/j.redox.2020.101605. PubMed link
  • Sorrentino A, Steinhorn B, Troncone L, Badole SL, Saravi SSS, Eroglu E, Kijewski MF, Divakaran S, Di Carli M, Michel T. Reversal of heart failure in a chemogenetic model of persistent cardiac redox stress. Am J Physiol Heart Circ Physiol. 2019 317: H617-H626. PMCID:PMC6766720.  PubMed link
  • Eroglu E, Saravi S, Sorrentino A, Steinhorn B, Michel T. Discordance between eNOS phosphorylation and activation revealed by multispectral imaging and chemogenetic methods.  Proc Natl Acad Sci USA 2019, 116:20210-20217. PMID: 31527268. PubMed link

We discovered that eNOS phosphorylation does not necessarily lead to enzyme activation.  For many years, we and many others had used eNOS phosphorylation as a surrogate for enzyme activation.  Using the hydrogen peroxide biosensor HyPer, we discovered a marked discordance between eNOS phosphorylation and increased NO generation in cultured endothelial cells.  See:

Eroglu E, Saravi S, Sorrentino A, Steinhorn B, Michel T. Discordance between eNOS phosphorylation and activation revealed by multispectral imaging and chemogenetic methods.  Proc Natl Acad Sci USA 2019, 116:20210-20217. PMID: 31527268.

Also check out our publication of a novel chemogenetic heart-failure model system published in Nature Communications:

Reversal of heart failure in a chemogenetic model of persistent cardiac redox stress

Andrea Sorrentino, Benjamin Steinhorn, Luca Troncone, Seyed Soheil Saravi, Sachin L Badole, Emrah Eroglu, Marie Foley Kijewski, Sanjay Divakaran, Marcelo Di Carli, Thomas Michel

Chemogenetic generation of hydrogen peroxide in the heart induces severe cardiac dysfunction

Benjamin Steinhorn, Andrea Sorrentino, Sachin Badole, Yulia Bogdanova, Vsevolod Belousov & Thomas Michel

Fig. 1

  • Steinhorn B, Sartoretto J, Sorrentino A, Kalwa H, Romero N, Abel ED, Michel T.  Insulin-dependent metabolic and inotropic responses in the heart are modulated by hydrogen peroxide from NADPH oxidase isoforms NOX2 and NOX4. Free Radic Biol Med. 2017, 113:16-25.
  • Tarrago L, Péterfi Z , Lee BC, Michel T, and Gladyshev V. Monitoring methionine sulfoxide with stereospecific mechanism-based fluorescent sensors. Nature Chem Biol 2015, 11: 332-338. PMID: 25799144.
  • Kalwa H, Sartoretto J, Martinelli R, Romero N, Steinhorn B, Tao M, Ozaki K, Carman C, Michel T A central role for hydrogen peroxide in P2Y1 ADP receptor-mediated cellular responses in vascular endothelium. Proc Nat Acad Sci USA 2014, Mar 4;111(9):3383-8. doi: 10.1073/pnas.1320854111.  PMID:24550450
  • Steinhorn B, Loscalzo J and Michel T. Rondo in E: Nitroglycerin, nitric oxide, and endothelial therapeutics.  New England Journal of Medicine  2015; 373:277-280.